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Solid Substrate Room Temperature Phosphorimetry for the Determination of Trace Mercury Based on the Reaction between Alkaline Phosphatase and Mercury Using Triton X‐100 as a Sensitizer
Author(s) -
Liu Jiaming,
Li Feiming,
Lin Shaoqin,
Li Zhiming,
Chen Yayu,
Lin Huiqing,
Huang Lichai,
Cai Jingping,
Chen Xuejuan
Publication year - 2009
Publication title -
chinese journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.28
H-Index - 41
eISSN - 1614-7065
pISSN - 1001-604X
DOI - 10.1002/cjoc.200990301
Subject(s) - chemistry , mercury (programming language) , detection limit , repeatability , reagent , dithizone , analytical chemistry (journal) , selectivity , spectrophotometry , chromatography , catalysis , biochemistry , computer science , programming language
A new solid substrate room temperature phosphorimetry (SSRTP) for the determination of trace mercury has been established, using Triton X‐100 as a sensitizer. The regression equation of working curve was Δ I p =11.40 m (Hg 2+ )+1.569 (ag·spot −1 , n =6, Δ I p = I p1 − I p2 , I p1 and I p2 referred to the phosphorescence intensity of the blank reagent and the test solution, respectively), and correlation coefficient ( r ) was 0.9984. The RSD valus of the determination of 0.016 and 8.0 ag·spot −1 Hg 2+ were 4.1% and 1.7% ( n =8), respectively, indicating that the method had good repeatability. The limit of detection (LOD) calculated by 3Sb/ k was 7.0 zg·spot −1 Hg 2+ (corresponding concentration: 1.8×10 −17 g·mL −1 , Sb=0.025, n =11). This method has high sensitivity, selectivity and precision, which was applied to determination of trace mercury in water samples with the result being agreed very well with that of dithizone extraction spectrophotometry.