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Synthesis and Application of Prenyl‐Derived Photoaffinity Probes
Author(s) -
LI Lingdong,
TANG Wei,
ZHAO Zongbao
Publication year - 2009
Publication title -
chinese journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.28
H-Index - 41
eISSN - 1614-7065
pISSN - 1001-604X
DOI - 10.1002/cjoc.200990233
Subject(s) - chemistry , biotin , photoaffinity labeling , streptavidin , click chemistry , biotinylation , azide , prenylation , combinatorial chemistry , saccharomyces cerevisiae , proteome , stereochemistry , biochemistry , binding site , organic chemistry , yeast , enzyme
Three photoaffinity probes containing isoprenoid chains and an azide group were synthesized using one‐pot coupling reaction as the key step. The capability of these probes as labeling agents for isoprenoid chain‐interacting proteins from Saccharomyces cerevisiae proteome was validated by photoaffinity reaction and "click" conjunction with the biotin reporter followed by streptavidin blot analysis.