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Synthesis, Characterization and Biological Activities of a New Fluorescent Indicator for the Intracellular Calcium Ions
Author(s) -
HE Huaizhen,
LEI Lei,
LI Jianli,
SHI Zhen
Publication year - 2009
Publication title -
chinese journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.28
H-Index - 41
eISSN - 1614-7065
pISSN - 1001-604X
DOI - 10.1002/cjoc.200990196
Subject(s) - chemistry , fluorescence , moiety , calcium , chelation , combinatorial chemistry , ion , proton nmr , biophysics , inorganic chemistry , stereochemistry , organic chemistry , physics , quantum mechanics , biology
A novel calcium‐selective fluorescent indicator Fluo‐3M AM was synthesized by introduction of a methyl group into the Ca 2+ ‐chelating moiety and adequately characterized by spectral methods ( 1 H NMR, GC‐MS, IR and MALDI‐TOF MS). Meanwhile, its fluorescence spectra and some biological activities have been also studied. The results indicate that the new fluorescent indicator has relatively high affinity to calcium and a strong fluorescence signal, which should be useful for biomedical researchers to investigate the effects of calcium ions in biosystems.