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Structure, DNA Binding Studies and Cytotoxicity of Complex [Pd(phen)( L ‐asp)]·3H 2 O
Author(s) -
GAO Enjun,
LIU Lei,
SHI Chunyue,
YIN Hongxi,
ZHU Mingchang,
WU Qiong,
LIU Qitao
Publication year - 2009
Publication title -
chinese journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.28
H-Index - 41
eISSN - 1614-7065
pISSN - 1001-604X
DOI - 10.1002/cjoc.200990177
Subject(s) - chemistry , hela , cytotoxicity , dna , cleave , stereochemistry , gel electrophoresis , crystal structure , crystallography , in vitro , biochemistry
The palladium(II) complex of [Pd(phen)( L ‐asp)]·3H 2 O (phen=1,10‐phenanthroline, H 2 L‐asp= L ‐aspartic acid) has been synthesized from a solution reaction and analyzed by elemental analyses, 1 H NMR and IR spectra. Moreover, the complex has been structurally characterized by single‐crystal X‐ray diffractometry. The cytotoxicity assay of the complex and cis ‐DDP as reference substance against three different cancer cell lines (Hela, Hep‐G2 and KB) has been conducted. The results show that the Pd complex exhibits higher cytotoxicity against Hela system. The study on the interaction of the Pd complex with fish sperm DNA (FS‐DNA) has been performed with diverse spectroscopic techniques, showing that the complex is bound to the fish sperm DNA via an intercalative mode. Gel electrophoresis assay demonstrates the ability of the complex to cleave the pBR 322 plasmid DNA.