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DNA‐binding Studies of Daunorubicin in the Presence of Methylene Blue by Spectroscopy and Voltammetry Techniques
Author(s) -
HAJIAN Reza,
SHAMS Nafiseh,
PARVIN Afsaneh
Publication year - 2009
Publication title -
chinese journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.28
H-Index - 41
eISSN - 1614-7065
pISSN - 1001-604X
DOI - 10.1002/cjoc.200990176
Subject(s) - chemistry , daunorubicin , methylene blue , intercalation (chemistry) , cyclic voltammetry , quenching (fluorescence) , differential pulse voltammetry , voltammetry , methylene , spectrophotometry , fluorescence spectroscopy , fluorescence , nuclear chemistry , photochemistry , inorganic chemistry , electrochemistry , electrode , chromatography , organic chemistry , medicine , physics , photocatalysis , quantum mechanics , leukemia , catalysis
The interaction of daunorubicin with calf thymus DNA has been investigated with the use of methylene blue dye as a spectral probe by the application of UV‐Vis spectrophotometry, spectrofluorometry and voltammetry. The voltammetric behavior of daunorubicin has been investigated at a glassy carbon electrode using cyclic and differential pulse voltammetry. Both UV‐vis spectrophotometry and cyclic voltammetry studies confirmed the intercalation reaction. The results showed that both daunorubicin and methylene blue molecules could intercalate into the double helix of DNA. The apparent binding constant of daunorubicin with DNA has been found to be 7.8 ×10 4 L·mol −1 . The fluorescence signal of daunorubicin and methylene blue was quenched with DNA addition. The Stern‐Volmer equation was plotted based on the quenching fluorescence signal of daunorubicin.