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Determination of Trace Protein by Methylene Blue‐Tetraphenylborate Fluorescence Probe Using Polyethylene Glycol as Sensitizer
Author(s) -
LIU Jiaming,
LI Zhiming,
HUANG Xiaomei,
ZENG Liqing,
CHEN Xiaohua,
SONG Dongqin,
ZHENG Zhenzhen
Publication year - 2009
Publication title -
chinese journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.28
H-Index - 41
eISSN - 1614-7065
pISSN - 1001-604X
DOI - 10.1002/cjoc.200990054
Subject(s) - chemistry , polyethylene glycol , fluorescence , detection limit , bovine serum albumin , tetraphenylborate , peg ratio , methylene blue , chromatography , ionic strength , reagent , nile blue , human serum albumin , buffer solution , linear range , quenching (fluorescence) , nuclear chemistry , aqueous solution , organic chemistry , ion , physics , finance , quantum mechanics , photocatalysis , economics , catalysis
An ionic association complex of methylene blue‐tetraphenylborate ([MB] + ·[B(C 6 H 5 ) 4 ] − ) can emit a strong and stable fluorescence in KH 2 PO 4 ‐Na 2 HPO 4 buffer solution. In the presence of bovine serum albumin (BSA), the fluorescence signal of [MB] + ·[B(C 6 H 5 ) 4 ] − can be sharply quenched, which can be further quenched by using polyethylene glycol (PEG) as a sensitizer where the Δ F (Δ F = F 0 − F , F 0 and F are the fluorescence intensities of the blank reagent and the test solution, respectively) of the system with PEG is 9.1 times higher than that without PEG, showing PEG has strong sensitizing effect on the quenching of a fluorescence signal. And there is a good linear correlation between Δ F and the content of BSA. Thus, a new fluorescence probe for the determination of trace protein has been established, with the linear range of 0.11–88.0 pg·L −1 and the detection limit of 22.0 ag·mL −1 BSA. This sensitive method has been applied to the determination of protein in human serum samples with satisfactory results. And the reaction mechanism was also discussed. Under the same condition, the new method can be used to determie not only BSA, human serum albumin (HAS), ovalbumin (OVA) and γ ‐globulin ( γ ‐G), respectively, but also the total protein in serum, brain and spinal cord.