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Square Wave Voltammetric Label‐free Determination of the Natural Protein Material Silk Fibroin
Author(s) -
MA MingMing,
SONG JunFeng
Publication year - 2008
Publication title -
chinese journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.28
H-Index - 41
eISSN - 1614-7065
pISSN - 1001-604X
DOI - 10.1002/cjoc.200890371
Subject(s) - fibroin , chemistry , square wave , detection limit , cyclic voltammetry , silk , electrochemistry , horizontal scan rate , analytical chemistry (journal) , voltammetry , nuclear chemistry , chromatography , electrode , composite material , physics , quantum mechanics , voltage , materials science
The electrochemical behavior of silk fibroin (SF) was investigated by cyclic voltammetry and square wave voltammetry in 0.01 mol/L HCl for the first time. Within the potential scan range of 0.0 to1.2 V (vs. SCE), two oxidative peaks at 0.91 V ( P a,1 ) and 0.43 V ( P a,2 ) as well as one reductive peak at 0.24 V ( P c ) were observed on cyclic voltammogram at scan rate of 0.2 V/s. The peak current of the peak P a,1 was linear with SF concentration in the range of 5.8×10 −8 to 1.1×10 −6 mol/L, with the limit of detection 3.0×10 −8 mol/L ( S / N =3). The proposed method was of high selectivity without the interferences from the coexisting substances such as another natural protein material sericin and other small molecular substances. It was applied to the determination of SF in raw silk liquid samples without any pre‐separation and pre‐purification.