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Resonance Rayleigh Scattering Spectrum of Palladium(II)‐ Clindamycin‐Halofluorescein Systems and Their Analytical Applications
Author(s) -
YI AoEr,
LIU ZhongFang,
LIU ShaoPu,
KONG Ling
Publication year - 2008
Publication title -
chinese journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.28
H-Index - 41
eISSN - 1614-7065
pISSN - 1001-604X
DOI - 10.1002/cjoc.200890338
Subject(s) - chemistry , erythrosine , eosin y , rayleigh scattering , analytical chemistry (journal) , fluorescence , cationic polymerization , resonance (particle physics) , absorption (acoustics) , absorption spectroscopy , spectral line , ternary numeral system , detection limit , buffer solution , chromatography , organic chemistry , physics , photocatalysis , quantum mechanics , astronomy , acoustics , optics , phase (matter) , catalysis , particle physics
In pH 5.0–5.4 HOAc‐NaOAc buffer solution, clindamycin (Clin) could react with Pd(II) to form a 1:1 cationic chelate, which could further react with halofluorescein dyes such as diiodofluorescein (DIF), erythrosine (Ery) and eosin Y (EY) to form 1:1 ion‐association complexes. As a result, not only the absorption and fluorescence spectra were changed, but also the resonance Rayleigh scattering (RRS) intensities enhanced greatly and new RRS spectra appeared. The three reaction products had characteristic RRS spectra and their maximum RRS wavelengths were located at 285 (DIF system), 287 (Ery system) and 321 (EY system) nm, respectively. The scattering intensities were proportional to the concentration of Clin in certain range which could be applied to determine Clin. The linear ranges and detection limits of Clin were 0.025–2.1 µg·mL −1 and 7.8 ng·mL −1 for the DIF system, 0.053–2.4 µg·mL −1 and 16.0 ng·mL −1 for the Ery system, 0.038–2.4 µg·mL −1 and 11.0 ng·mL −1 for the EY system, respectively. In this work, the optimum reaction conditions and the foreign substances were investigated. A simple, sensitive and fast method was developed for the determination of Clin either in the pharmaceutical form or in the human body fluid. Moreover, the composition, structure, reaction mechanism of the ternary complexes and their effects on the absorption, fluorescence and RRS spectra as well as the reasons of RRS enhancement were discussed.