Premium
Chiral Separation of Novel α ‐Aminophosphonates Containing a Benzothiazole Moiety by Liquid Chromatography Using an Amylose Stationary Phase
Author(s) -
LU Ping,
ZHANG YuPing,
SONG BaoAn,
YANG Song,
BHADURY Shankar Pinaki,
HU DeYu,
XUE Wei,
CHEN Zhuo,
JIN LinHong
Publication year - 2008
Publication title -
chinese journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.28
H-Index - 41
eISSN - 1614-7065
pISSN - 1001-604X
DOI - 10.1002/cjoc.200890300
Subject(s) - chemistry , enantiomer , chromatography , benzothiazole , moiety , amylose , resolution (logic) , high performance liquid chromatography , analyte , chiral column chromatography , chiral stationary phase , organic chemistry , artificial intelligence , starch , computer science
The present report describes a chiral HPLC method for the enantiomeric separation of α ‐aminophosphonate derivatives using two new coated and immobilized amylose‐based chiral stationary phases (CSP, Chiralpak IA and Chiralpak AD‐H). The chromatographic parameters such as retention factor ( k ), separation factor ( α ), and resolution ( R s ) of the solutes were investigated on these two CSPs. Reasonably good baseline separation for these compounds was achieved using Chiralpak IA column. The influences of temperature, content of ethanol modifier and the structure of analyte were also studied. THF, EtOAc and CH 2 Cl 2 were used as eluents on analytical and semi‐preparative columns. Highly enriched enantiomers with purities of up to 96.4% –100% ( ee ) and yields of 90.2% –95.5% were obtained, respectively. The proposed methods were found to be suitable and accurate for rapid separation and semi‐preparation of enantiomeric α ‐aminophosphonate derivatives available.