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Detection and Sequence Identification of Dinucleotides Produced from N ‐Phosphoryl Alanine and Four Nucleosides by HPLC‐ESI‐MS/MS
Author(s) -
GUO YanChun,
CAO ShuXia,
LIAO XinCheng,
ZHAO YuFen
Publication year - 2008
Publication title -
chinese journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.28
H-Index - 41
eISSN - 1614-7065
pISSN - 1001-604X
DOI - 10.1002/cjoc.200890233
Subject(s) - chemistry , guanosine , uridine , cytidine , high performance liquid chromatography , fragmentation (computing) , nucleotide , alanine , stereochemistry , sequence (biology) , mass spectrometry , inosine , chromatography , adenosine , biochemistry , amino acid , enzyme , rna , computer science , gene , operating system
The products of the model reaction between N ‐( O , O ‐diisopropyl phosphoryl) alanine (DIPP‐Ala) and four nucleosides (adenosine, uridine, cytidine and guanosine) were studied by HPLC‐ESI‐MS/MS. The results showed that different mononucleotides and dinucleotides were produced. The sequences of dinucleotides formed from the DIPP‐Ala and four nucleosides were identified. The results revealed that the c ‐ions from backbone fragmentation of the dinucleotides could be considered as the sequence‐diagnostic ions. It was firstly testified that the diagnostic c ‐ions in positive and negative modes could be applied to identify the sequence of dinucleotide products formed in the reaction system.