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Purification of Biliary Protein and Its Effect on Calcium Carbonate Mineralization
Author(s) -
YAO ChengLi
Publication year - 2008
Publication title -
chinese journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.28
H-Index - 41
eISSN - 1614-7065
pISSN - 1001-604X
DOI - 10.1002/cjoc.200890190
Subject(s) - chemistry , calcium carbonate , calcium , scanning electron microscope , mineralization (soil science) , fourier transform infrared spectroscopy , crystallography , chemical engineering , organic chemistry , physics , quantum mechanics , nitrogen , engineering
The biliary protein (BP) was isolated from pig bile by gel filtration. The interaction between Ca 2+ and protein was measured by fluorescence spectra. The result showed that there was a strong coordination between biliary protein and Ca 2+ . The CaCO 3 crystals obtained in systems with and without BP were characterized by scanning electron microscopy, Fourier transform infrared spectrography and powder X‐ray diffractometry. The possible formation mechanism of CaCO 3 in biliary protein solution was discussed.