Purification of Biliary Protein and Its Effect on Calcium Carbonate Mineralization | Zendy

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Purification of Biliary Protein and Its Effect on Calcium Carbonate Mineralization

Author(s) -

YAO ChengLi

Publication year - 2008

Publication title -

chinese journal of chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.28

H-Index - 41

eISSN - 1614-7065

pISSN - 1001-604X

DOI - 10.1002/cjoc.200890190

Subject(s) - chemistry , calcium carbonate , calcium , scanning electron microscope , mineralization (soil science) , fourier transform infrared spectroscopy , crystallography , chemical engineering , organic chemistry , physics , quantum mechanics , nitrogen , engineering

The biliary protein (BP) was isolated from pig bile by gel filtration. The interaction between Ca 2+ and protein was measured by fluorescence spectra. The result showed that there was a strong coordination between biliary protein and Ca 2+ . The CaCO 3 crystals obtained in systems with and without BP were characterized by scanning electron microscopy, Fourier transform infrared spectrography and powder X‐ray diffractometry. The possible formation mechanism of CaCO 3 in biliary protein solution was discussed.

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