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Platinum Nanoparticles Modified Electrode for Rapid Electrochemical Detection of Escherichia coli
Author(s) -
CHENG YuXiao,
LIU YaJun,
HUANG JingJing,
FENG Zhen,
XIAN YueZhong,
WU ZiRong,
Zhang Wen,
JIN LiTong
Publication year - 2008
Publication title -
chinese journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.28
H-Index - 41
eISSN - 1614-7065
pISSN - 1001-604X
DOI - 10.1002/cjoc.200890059
Subject(s) - detection limit , platinum nanoparticles , chemistry , escherichia coli , electrode , platinum , electrochemistry , nanoparticle , nuclear chemistry , nanotechnology , chromatography , catalysis , biochemistry , materials science , gene
A platinum nanoparticles chemically modified electrode (PtNP/GCE) was fabricated by electro‐deposition and used to detection of Escherichia coli ( E. coli ). The detection principle was based on determination of p ‐aminophenol that was produced by an enzymatic reaction in E. coli solution. The sensitivity of the detection was improved by the platinum nanoparticle modified electrode and optimization of the detection system. The current responses were proportional to the density of E. coli ranging from 50 to 1.0×10 5 cfu/mL, with detection limit of 20 cfu/mL, and the detection time was less than 4 h. Compared with conventional methods, the electrochemical technology described here could be suitable for rapid detection of E. coli in the fields of food industry, environmental monitoring and clinic biomedicine.