C 60 ‐Based Ebselen Derivative: Synthesis and Enhanced Protective Effect on Mouse Thymus Cells

A C 60 ‐based ebselen derivative 3 was synthesized through a Bingel cyclopropanation of C 60 with the ebselen malonate 2 . It was obtained in a three‐step synthesis starting from 2‐(chloroseleno)benzoyl chloride and 2‐(2‐aminoethoxy)ethanol, in a 42% yield (based on consumed C 60 ). Its structure was characterized by 1 H NMR, 13 C NMR, IR, FAB‐MS, and elemental analyses techniques. To verify that the C 60 ‐based ebselen derivative 3 had enhanced effect on viability of mouse thymus cells, the C 60 derivative 4 and ebselen derivative 2 were selected to treat the mouse thymus cells using the same procedures as those with the C 60 ‐based ebselen derivative 3 . The result shows that MTT(OD) values of compound 3 treated groups (0.335±0.021) were all higher than those of compound 4 (0.283±0.031) and compound 2 (0.247±0.025) treated groups, indicating that the compound 3 has an advantage over compounds 2 and 4 in promoting the viability of the mouse thymus cell.