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Probing Cellular Binding of Dendrofullerene by in‐situ Electrochemical Contact Angle Measurement
Author(s) -
ZHOU Jian,
ZHANG RenYun,
WU ChunHui,
ZHAO XueYan,
ZHENG LiQiang,
FU DeGang,
CHEN BaoAn,
WANG XueMei
Publication year - 2008
Publication title -
chinese journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.28
H-Index - 41
eISSN - 1614-7065
pISSN - 1001-604X
DOI - 10.1002/cjoc.200890003
Subject(s) - contact angle , chemistry , electrochemistry , membrane , in situ , biophysics , electrochemical cell , permeation , cancer cell , fluorescence microscope , intracellular , k562 cells , fluorescence , nanotechnology , combinatorial chemistry , cell , chemical engineering , organic chemistry , biochemistry , cancer , electrode , materials science , optics , medicine , physics , engineering , biology
Abstract Dendrofullerene (C 60 DF) is a novel fullerene derivative with potential and promising biomedical applications. In this work, electrochemical/contact angle behavior of C 60 DF in the cellular system has been explored by in‐situ electrochemical contact angle measurement. This measuring system is a newly developed technique which can provide electrochemical and contact angle detection simultaneously. The electrochemical results indicate that dendrofullerene may effectively bind and permeate the tumor cell membrane and then distribute into the cancer cells. Our observations of in‐situ electrochemical contact angle measurement also illustrate that the permeation and interaction of C 60 DF with target cancer cells may lead to some variation of the configurational structure of the relative cell membrane and thus result in the change of hydrophilic/hydrophobic properties of target cellular system. Furthermore, through confocus fluorescence microscopy study we found that, upon application of C 60 DF, the intracellular accumulation of anticancer drug daunorubicin in leukemia K562 cells could be remarkably enhanced by C 60 DF. Therefore fullerene derivatives were demonstrated to be a good candidate that can play an important role in improving the intracellular drug uptake in the target cancer cells.