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A New Nanogold‐Labeled Immunoresonance Scattering Spectral Probe for Determination of Trace IgG
Author(s) -
Jiang ZhiLiang,
Li Yan,
Sun ShuangJiao,
Chen Bing
Publication year - 2007
Publication title -
chinese journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.28
H-Index - 41
eISSN - 1614-7065
pISSN - 1001-604X
DOI - 10.1002/cjoc.200790238
Subject(s) - chemistry , trisodium citrate , detection limit , polyethylene glycol , colloidal gold , peg ratio , immunoglobulin g , scattering , analytical chemistry (journal) , chromatography , nuclear chemistry , nanoparticle , antibody , nanotechnology , biochemistry , optics , materials science , physics , finance , biology , immunology , economics
A kind of 9 nm gold nanoparticles was prepared with the trisodium citrate and used to label goat anti‐human IgG to obtain an IgG immunoresonance scattering spectral probe. In pH 5.8 buffer solution and in the presence of polyethylene glycol (PEG), the immune reaction between gold‐labeled goat anti‐human IgG and IgG took place, and the resonance scattering intensity at 580 nm ( I 580 nm ) was enhanced greatly. The enhanced intensity ΔIRS is proportional to the IgG concentration from 1.3 to 1.5×10 3 ng·mL −1 , with a detection limit of 0.78 ng·mL −1 . This assay showed high sensitivity and good selectivity for quantitative determination of IgG in human serum, with satisfactory results.