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A Highly Sensitive Spectrophotometric Assay of Bleomycins Based on the Fading Reaction of Some Halofluorescein Dyes
Author(s) -
Liu JiangTao,
Liu ZhongFang,
Hu XiaoLi,
Kong Ling,
Liu ShaoPu
Publication year - 2007
Publication title -
chinese journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.28
H-Index - 41
eISSN - 1614-7065
pISSN - 1001-604X
DOI - 10.1002/cjoc.200790216
Subject(s) - chemistry , absorbance , rose bengal , eosin y , fading , spectrophotometry , bleomycin , eosin , nuclear chemistry , chromatography , organic chemistry , staining , medicine , telecommunications , decoding methods , photocatalysis , pathology , computer science , catalysis , surgery , chemotherapy
In weak acidic medium, anticancer antibiotics bleomycin A 5 (BLMA 5 ) and bleomycin A 2 (BLMA 2 ) can react with halofluorescein dyes such as erythrosin (Ery), eosin Y (EY), eosin B (EB) and rose bengal (RB) by virtue of electrostatic attraction and hydrophobic force to form the ion‐association complexes, which can result in the fading reactions of four halofluorescein dyes. The maximum fading wavelengths of these four dyes were located at 527 nm for Ery, 515 nm for EY, 517 nm for EB and 546 nm for RB, respectively. The decrements of absorbance (( A ) were directly proportional to the concentrations of bleomycin in a certain range. A new method for the determination of bleomycins anticancer drugs based on fading reactions of halofluorescein dyes has been developed. The method was not only highly sensitive but also simple and rapid. The molar absorptivities ( ε ) ranged from 1.5×10 5 to 7.5×10 5 L·mol −1 ·cm −1 . It was applied to determination of the bleomycins in human serum, urine and rabbit serum samples. In this work, the spectral properties and the optimum reaction conditions were investigated. The structure of ion‐association complexes and the reaction mechanism were discussed.