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Study on the Supramolecular Interaction of β ‐Cyclodextrin with Gemfibrozil by Spectrofluorimetry and Its Analytical Application
Author(s) -
Huang Hui,
Liu Fang,
Jia BaoXiu,
Xu KeHua,
Chen ZhenZhen,
Tang Bo
Publication year - 2007
Publication title -
chinese journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.28
H-Index - 41
eISSN - 1614-7065
pISSN - 1001-604X
DOI - 10.1002/cjoc.200790065
Subject(s) - chemistry , gemfibrozil , cyclodextrin , supramolecular chemistry , aqueous solution , binding constant , chromatography , detection limit , fluorescence , analytical chemistry (journal) , organic chemistry , molecule , binding site , biochemistry , physics , quantum mechanics , cholesterol
The supramolecular interaction of gemfibrozil with β ‐cyclodextrin ( β ‐CD) was studied by spectrofluorimetry. The mechanism of the inclusion was discussed by spectrofluoremetry, infrared spectrum and 1 H NMR spectrum. The results showed that a 1:1 ( β ‐CD:gemfibrozil) complex was formed with an apparent association constant of 3.844×10 3 L·mol −1 . Based on the enhancement of the fluorescent intensity of gemfibrozil, a spectrofluorimetric method for the determination of gemfibrozil in bulk aqueous solution in the presence of β ‐CD was developed. The linear range was 3.30 ng·mL −1 –6.00 µg·mL −1 with the detection limit of 0.980 ng·mL −1 . There was no interference from the excipients normally used in tablet composition and the serum main compositions. The proposed method was then successfully applied to the determination of gemfibrozil in capsules and serum.