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Development of an RPLC‐based Method for Measurement of Indoprofen Stability and Validation of the Method
Author(s) -
Liou YiBo,
Chen FuAn,
Lee JenAi,
Ho HsiuO,
Wu AnBang
Publication year - 2006
Publication title -
chinese journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.28
H-Index - 41
eISSN - 1614-7065
pISSN - 1001-604X
DOI - 10.1002/cjoc.200690294
Subject(s) - chemistry , chromatography , degradation (telecommunications) , decomposition , resolution (logic) , selectivity , pharmaceutical formulation , analytical chemistry (journal) , organic chemistry , telecommunications , artificial intelligence , computer science , catalysis
A method for quantitative measurement of the photochemical decomposition of the anti‐inflammatory agent, indoprofen (INP) is descriped. An RPLC‐based assay that could determine the extent of degradation of INP in a rapid, sensitive, and accurate manner was developed. The method was validated under photoirradiation. Quantitation was monitored with an Inertsil ODS‐3V column using a mobile phase of acetonitril and 1% HOAc solution in deionized H 2 O. Statistics relevant to the system criteria, peak integrity and resolution among the parent drug and its degradation products were performed. From the intra‐ and inter‐day tests, the coefficients of variation were found to range from 0.59% to 4.25% for the former and from 0.71% to 4.86% for the latter. The good selectivity and specificity of this RPLC‐based procedure render it suitable for measurements of INP stability.