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Determination of Four Active Ingredients in Vc Yinqiao Tablets by Capillary Zone Electrophoresis with Amperometric Detection †
Author(s) -
LÜ Jin,
Wang QingJiang,
Cheng Xi,
Liu HaiYan,
He PinGang,
Fang YuZhi
Publication year - 2006
Publication title -
chinese journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.28
H-Index - 41
eISSN - 1614-7065
pISSN - 1001-604X
DOI - 10.1002/cjoc.200690210
Subject(s) - chemistry , chlorogenic acid , capillary electrophoresis , amperometry , chromatography , reproducibility , caffeic acid , detection limit , ascorbic acid , analytical chemistry (journal) , electrode , electrochemistry , biochemistry , food science , antioxidant
A simple, reliable and reproducible method, based on capillary zone electrophoresis with amperometric detection (CZE‐AD), has been developed for simultaneous determination of four active ingredients in Vc Yinqiao tablets including paracetamol, vitamin C, caffeic acid and chlorogenic acid. A carbon‐disk electrode was used as working electrode and 0.95 V (versus SCE) was selected as detection potential. The optimal conditions of CZE experiment were 30 mmol·L −1 borate solution (pH 9.5) as running buffer, 14 kV as separation voltage and 8 s (14 kV) as electro‐kinetic sampling time. Under the selected optimum conditions, paracetamol, vitamin C, caffeic acid and chlorogenic acid could be perfectly separated within 22 min, and their detection limits ( S/N =3) ranged from 5×10 −7 to1×10 −6 mol·L −1 . This proposed method demonstrated good reproducibility with relative standard deviations of less than 3% for both migration time and peak current ( n =7). The utility of this method was demonstrated by monitoring a kind of compound medicine named Vc Yinqiao tablets and the assay results were satisfactory.