Microcalorimetric Study on the Effect of Sodium Arsenite on Metabolic Activity of Mitochondria Isolated from  Carassius auratus  Liver Tissue | Zendy

Dai Jie | Zendy; Liu Yi | Zendy; Zhu JunCheng | Zendy; Zhang YeZhong | Zendy

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Microcalorimetric Study on the Effect of Sodium Arsenite on Metabolic Activity of Mitochondria Isolated from Carassius auratus Liver Tissue

Author(s) -

Dai Jie,

Liu Yi,

Zhu JunCheng,

Zhang YeZhong

Publication year - 2006

Publication title -

chinese journal of chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.28

H-Index - 41

eISSN - 1614-7065

pISSN - 1001-604X

DOI - 10.1002/cjoc.200690207

Subject(s) - chemistry , sodium arsenite , isothermal microcalorimetry , ampoule , sodium , mitochondrion , carassius auratus , metabolic rate , arsenite , chromatography , biochemistry , enthalpy , thermodynamics , endocrinology , organic chemistry , medicine , physics , arsenic , fishery , biology , fish <actinopterygii>

Metabolic thermogenic curves of mitochondria isolated from liver tissue of Carassius auratus and the effect of different concentration of NaAsO 2 on it were investigated by TAM air isothermal microcalorimeter, ampoule method, at 28.00 °C. From the thermogenic curves, activity recovery rate constants k , the maximum heat production rate ( P max ) and the total heat produced ( Q ) were obtained. The values of k and P max decline gradually with the increase of the concentration of NaAsO 2 , and both of the values of P max and k are highly correlated to the concentration of NaAsO 2 . When concentration of NaAsO 2 reached 16.0 µg/mL, the maximum heat production rate dropped to 70.8% of the control group, and the corresponding percentage of k was 71.4% of the control group. This experimental result indicates that the addition of NaAsO 2 has restrained the metabolic activities of mitochondria in vitro .

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