Premium
Study on Interaction of Ginsenosides with Bovine or Human Serum Albumin Using Wavelength Modulation Surface Plasmon Resonance Biosensor
Author(s) -
Liu Xia,
Sun Ying,
Song DaQian,
Li XuWen,
Zhang QingLin,
Tian Yuan,
Liu ZhongYing,
Zhang HanQi
Publication year - 2006
Publication title -
chinese journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.28
H-Index - 41
eISSN - 1614-7065
pISSN - 1001-604X
DOI - 10.1002/cjoc.200690126
Subject(s) - chemistry , surface plasmon resonance , biosensor , bovine serum albumin , human serum albumin , serum albumin , chromatography , modulation (music) , biophysics , biochemistry , nanotechnology , nanoparticle , materials science , philosophy , aesthetics , biology
To use a newly developed wavelength modulation surface plasmon resonance (SPR) biosensor, an experimental protocol was developed to investigate the interaction of ginsenosides with serum albumin. With a known concentration of the ginsenosides, bound percentages of the ginsenosides with human serum albumin (HSA) or bovine serum albumin (BSA) were obtained. SPR technique could require no labeling and this method provided the detailed information on association and disassociation of molecules in real time. The results indicate that the sensitivity of wavelength modulation SPR biosensor is sufficient for detection and characterization of binding events involving low‐molecular weight compounds and their immobilized protein targets.