Premium
Investigation of the Inhibition of Cyanide on Metabolism of Fish Liver Mitochondria by Microcalorimetry
Author(s) -
Li HuiRong,
Liu Yi,
Dai Jie,
Qin CaiQin,
Zhang ZhongHai,
Qu SongSheng
Publication year - 2006
Publication title -
chinese journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.28
H-Index - 41
eISSN - 1614-7065
pISSN - 1001-604X
DOI - 10.1002/cjoc.200690120
Subject(s) - cyanide , chemistry , isothermal microcalorimetry , cyprinus , metabolism , respiration , mitochondrion , biochemistry , fish <actinopterygii> , environmental chemistry , organic chemistry , botany , biology , fishery , physics , quantum mechanics , enthalpy
A microcalorimetric technique based on the metabolic heat‐output was explored to evaluate the inhibition of cyanide on the mitochondrial metabolism of aquatic animal, Cyprinus carpio . The power‐time curves could be divided into four parts:lag phase, active recovery phase, stationary phase, and decline phase, and the corresponding thermokinetic parameters were obtained. The maximum heat production rate P max decreased in a linear manner with the increase of concentration of cyanide, however, such mitochondria of aquatic animal were still metabolized actively even under the action of high concentration of cyanide. All the observations suggested that the mitochondria of this aquatic animal should exhibit considerable ability of cyanide‐resistant respiration.