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Microcalorimetric Study of Energy Metabolism of HSV‐2 and FMDV Infection Processes
Author(s) -
Zhang Heng,
Zheng CongYi,
Li Jie,
Gu ChaoJiang,
Wang CunXin,
Liu YuWen
Publication year - 2006
Publication title -
chinese journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.28
H-Index - 41
eISSN - 1614-7065
pISSN - 1001-604X
DOI - 10.1002/cjoc.200690035
Subject(s) - metabolism , chemistry , isothermal microcalorimetry , infectivity , cell metabolism , hela , energy metabolism , interferon , virus , cell , microbiology and biotechnology , virology , biochemistry , biology , physics , quantum mechanics , enthalpy , endocrinology
The metabolic thermogenic power data of the HSV‐2 infected HeLa cells and the FMDV infected BHK‐21 cells were determined by LKB‐2277 bioactivity monitor. The aim of the study was to investigate the difference of the cell metabolism under the action of two different viruses and the effects of hyperthermia and drugs on it. The results illustrated that the metabolic thermogenic power of infected cells was larger than the uninfected ones and there was a significant difference between the metabolism heat released by the two types of infected cells. From the maximal thermal power and total metabolism heat, the infection process was observed to be thermosensitive and could be inhibited by interferon. Our experiments also revealed that 6 month storage of FMDV could attenuate its virulence and infectivity. The study shows that microcalorimetry is a potent tool to investigate the metabolism of virus infection process.