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Preparation of Nanosized CoHCF Modified Electrode and Its Application to Electroanalysis of Hemoglobin
Author(s) -
MeiChuan Liu,
Ping Li,
Li Zhang,
YueZhong Xian,
HongChun Ding,
ChengLin Zhang,
FenFen Zhang,
LiTong Jin
Publication year - 2005
Publication title -
chinese journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.28
H-Index - 41
eISSN - 1614-7065
pISSN - 1001-604X
DOI - 10.1002/cjoc.200590983
Subject(s) - chemistry , electrochemistry , detection limit , electrode , hemoglobin , nanoparticle , analytical chemistry (journal) , glassy carbon , linear range , nuclear chemistry , chromatography , nanotechnology , cyclic voltammetry , organic chemistry , materials science
A cobalt hexacyanoferrate (CoHCF) nanoparticle (size ca . 60 nm) chemically modified electrode (CME) was fabricated and the electrochemical behavior of hemoglobin (Hb) at this nanosized CoHCF CME was studied. In comparison with a bare glassy carbon electrode (GCE) and a general CoHCF CME electrodeposited in a traditional manner, the present nanosized CoHCF CME performed efficiently electrocatalytic reduction for Hb with relatively high sensitivity, stability, and longlife. Combined with liquid chromatography (LC), the nanosized CoHCF CME was used as the electrochemical detector of Hb in the established flow injection analysis‐electrochemical determination (FIA‐ECD) system. The peak current was a linear function of concentrations in the range from 2.5×10 ‐8 to 5.0×10 ‐6 mol/L for Hb, with detection limit of 1.4×10 ‐8 mol/L. The FIA‐ECD system has been successfully applied to assess the Hb content of clinic blood samples with advantages of sensitiveness, speediness, easy control and small sample‐consumption.