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An in vitro Study on Transition from NO‐Hemoglobin to Methemoglobin: Oxygen Effect
Author(s) -
Jiong Ma,
Yan Cen,
JiYao Chen
Publication year - 2005
Publication title -
chinese journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.28
H-Index - 41
eISSN - 1614-7065
pISSN - 1001-604X
DOI - 10.1002/cjoc.200590637
Subject(s) - methemoglobin , chemistry , hemoglobin , oxygen , nitric oxide , electron paramagnetic resonance , nitrosylation , biochemistry , hemeprotein , molecule , heme , nuclear magnetic resonance , organic chemistry , enzyme , physics
The nitrosyl‐hemoglobin (HbNO) is the carrier of nitric oxide (NO) which is the important messenger molecule displaying multiple physiologic and pathophysiologic roles. However it is still not clear for the fate of HbNO molecules during the venous‐arterial transit. In this letter, the HbNO transition in vitro was studied by using the electron paramagnetic resonance (EPR) spectra. It was found that HbNO molecules were stable when oxygen did not exist in the system but not stable in aerobic conditions. The absorption spectra further revealed that the methemoglobin (metHb) was the product of HbNO in aerobic environment, showing that the HbNO changed to metHb when there were enough oxygen molecules in the system.