Premium
Study on the Binding Mode of a Co(II) Complex with DNA
Author(s) -
QingHua Zhou,
Pin Yang
Publication year - 2005
Publication title -
chinese journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.28
H-Index - 41
eISSN - 1614-7065
pISSN - 1001-604X
DOI - 10.1002/cjoc.200590521
Subject(s) - chemistry , dna , binding constant , viscosity , amine gas treating , cleave , electrophoresis , gel electrophoresis , fluorescence , polyelectrolyte , binding site , analytical chemistry (journal) , chromatography , organic chemistry , biochemistry , thermodynamics , physics , quantum mechanics , polymer
Abstract The mode of binding of CoLCl 2 , here L=bis(2‐benzimidazolylmethyl)amine, with calf thymus DNA has been investigated by fluorescence measurements, equilibrium dialysis, viscosity experiments and gel electrophoresis. The complex was found to bind but weakly to DNA, with binding constant of 1.96×10 4 L/mol determind at 20 °C in a solution containing 5 mmol/L Tris‐HCl (pH 7.1) and 50 mmol/L NaCl. Polyelectrolyte theory was applied to analyse these values. Viscosity experiments show that binding did not alter the relative viscosity of DNA with any complexes to an appreciable extent. Electrophoresis test displayed that the compound could not cleave the DNA. These results show that the complex is essentially electrostatically bound to DNA.