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Fluorescence Study on the Interaction between Naproxen and Yeast DNA
Author(s) -
Ye BaoFen,
Zhang ZhiJie,
Ju HuangXian
Publication year - 2005
Publication title -
chinese journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.28
H-Index - 41
eISSN - 1614-7065
pISSN - 1001-604X
DOI - 10.1002/cjoc.200590013
Subject(s) - chemistry , quenching (fluorescence) , fluorescence , naproxen , yeast , dna , binding constant , base pair , analytical chemistry (journal) , binding site , biophysics , photochemistry , chromatography , biochemistry , medicine , physics , alternative medicine , pathology , quantum mechanics , biology
The interaction between naproxen and yeast DNA was studied using fluorescence spectrometry and ultra‐violet (UV) spectrometry. The addition of yeast dsDNA to naproxen solution resulted in a strong fluorescence quenching. Both the Stern‐Volmer and the Scatchard plots of the fluorescence quenching showed a curve with two slopes and a threshold DNA concentration of about 100 µmol · L −1 . The interaction between naproxen and the dsDNA was found to be a groove binding mode by UV spectra, the influence of ion strength and I − quenching effect. This mode was independent on DNA concentration. However, the increase in DNA concentration changed the binding constant K of naproxen to yeast dsDNA and the number of binding sites in DNA base pairs ( n ).