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Study on PEG‐(NH 4 ) 2 SO 4 aqueous two‐phase system and distribution behavior of drugs
Author(s) -
Li Lei,
He ChiYang,
Li SheHong,
Liu Feng,
Su Shun,
Kong XiangXu,
Li Na,
Li KeAn
Publication year - 2004
Publication title -
chinese journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.28
H-Index - 41
eISSN - 1614-7065
pISSN - 1001-604X
DOI - 10.1002/cjoc.20040221120
Subject(s) - chemistry , aqueous solution , polyethylene glycol , extraction (chemistry) , peg ratio , hydrogen bond , aqueous two phase system , chromatography , procaine hydrochloride , phase (matter) , elution , nuclear chemistry , procaine , organic chemistry , molecule , finance , economics , medicine , anesthesia
The distribution behavior of chlorpromazine hydrochloride (CPZ), procaine hydrochloride (PCN) and procaine amide hydrochloride (PCNA) in polyethylene glycol (PEG800 or PEG1500)‐(NH 4 SO 4 aqueous two‐phase systems has been investigated. The result shows that the PEG‐(NH 4 SO 4 aqueous two‐phase system has potential extraction capability in small molecular drug separation. In PEG800–(NH 4 ) 2 SO 4 system, the extraction efficiencies ( E ) of CPZ, PCN and PCNA amount to 92.8%, 74.5% and 74.4%, respectively, with the distribution coefficients ( K D ) being 25.7, 5.9 and 5.8, correspondingly. In PEG1500–(NH 4 ) 2 SO 4 system, the extraction efficiencies ( E ) of CPZ, PCN and PCNA are 93.7%, 71.3% and 63.24, respectively, with distribution coefficients ( K D ) of 39.6, 6.6 and 5.0, correspondingly. Based on the study on ultraviolet and fluorescence spectra and also distribution behavior of the drugs in PEG‐(NH 4 )SO 4 aqueous two‐phase system, extraction mechanism was further proposed that both hydrogen bond and hydrophobic interaction are involved in extraction.