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Elucidation of O ‐phosphoryl and N ‐phosphoryl amino acids by electrospray ionization tandem mass spectrometry
Author(s) -
Zhang JianChen,
Cao ShuXia,
Xu Jun,
Liao XinCheng,
Zhao YuFen
Publication year - 2004
Publication title -
chinese journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.28
H-Index - 41
eISSN - 1614-7065
pISSN - 1001-604X
DOI - 10.1002/cjoc.20040220822
Subject(s) - chemistry , electrospray ionization , tandem mass spectrometry , mass spectrometry , fragmentation (computing) , amino acid , protein mass spectrometry , electrospray , chromatography , biochemistry , computer science , operating system
Mass spectroscopic characteristics of phosphoryl amino acids were studied in detail by positive and negative electrospray ionization mass spectrometry (ESI‐MS) in conjunction with tandem mass spectrometry (MS/MS). Besides N ‐diisopropyloxyphosphoryl amino acids ( N ‐DIPP‐AA), O ‐phospho‐ and O ‐diisopropyloxyphosphoryl amino acids ( O ‐DIPP‐AA) were studied and compared to N ‐DIPP‐AA. The fragmentation pathways of [M+H] + , [M+Na] + and [M−H] − ions of phosphoryl amino acids were summarized. In addition to several similar patterns. each of them showed its characteristic fragmention.