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Enantiomeric separation of epinephrine and salbutamol by micellar electrokinetic chromatography using β‐cyclodextrin as chiral additive
Author(s) -
Zheng YanPeng,
Mo JinYuan
Publication year - 2004
Publication title -
chinese journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.28
H-Index - 41
eISSN - 1614-7065
pISSN - 1001-604X
DOI - 10.1002/cjoc.20040220816
Subject(s) - chemistry , enantiomer , micellar electrokinetic chromatography , chromatography , electrokinetic phenomena , cyclodextrin , ammonium chloride , capillary electrophoresis , organic chemistry
Enantiomeric separations of epinephrine and salbutamol, by means of micellar electrokinetic chromatography (MEKC) employing β‐cyclodextrin as chiral additive in ammonium chloride‐ammonia solution were investigated. In this system, the analytes migrated with the micellar phase towards the anode and were detected by electrochemistry using gold microelectrode at +0.65 V vs. SCE. The success of the chiral separations is strongly dependent on the concentration of β‐CD and SDS, and the optimal concentration is 8 mmol·L −1 and 15 mmol·L −1 respectively. The effects of detection potential, pH value of electrolyte and applied voltage were discussed also. Using the proposed method, baseline separation of the enantiomers could be accomplished in 6 min. Further, an attempt was made to elucidate the plausible mechanism of the chiral recognition.