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Amplified DNA detection sensitivity using streptavidin‐biotinylated protein complex: characterization by electrochemical impedance spectroscopy
Author(s) -
Cheng ZhiLiang,
Yang Fan,
Huang HaiZhen,
Yang XiuRong
Publication year - 2003
Publication title -
chinese journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.28
H-Index - 41
eISSN - 1614-7065
pISSN - 1001-604X
DOI - 10.1002/cjoc.20030211022
Subject(s) - streptavidin , chemistry , biotinylation , biosensor , oligonucleotide , sensitivity (control systems) , dielectric spectroscopy , nanotechnology , biotin , dna , combinatorial chemistry , electrochemistry , electrode , biochemistry , electronic engineering , materials science , engineering
Thiol‐terminated oligonucleotide was immobilized to gold surface by self‐assembly method. A novel amplification strategy was introduced for improving the sensitivity of DNA hybridization using biotin labeled protein‐streptavidin network complex. This complex can be formed in a cross‐linking network of molecules so that the amplification of the response signal will be realized due to the big molecular size of the complex. It could be proved from the impedance technique that this amplification strategy caused dramatic improvement of the detection sensitivity. These results give significant advances in the generality and sensitivity as it is applied to biosensing.