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Prediction of Retention Time of Solutes under Linear Gradient Elution Conditions in RP‐HPLC
Author(s) -
GengLiang Yang,
BaoJuan Tian,
Yi Chen,
DeXian Wang
Publication year - 2003
Publication title -
chinese journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.28
H-Index - 41
eISSN - 1614-7065
pISSN - 1001-604X
DOI - 10.1002/cjoc.20030210821
Subject(s) - chemistry , elution , gradient elution , retention time , chromatography , dwell time , vanillin , high performance liquid chromatography , phenanthrene , biphenyl , environmental chemistry , organic chemistry , medicine , clinical psychology
A model for predicting retention tune of solutes under linear gradient elution conditions has been established. In this model, the theoretical expressions under different elution modes were derived and tested with the retention behaviors of p ‐hydroxybenzaldehyde, vanillin, biphenyl, phenanthrene in gradient elution. With taking into account the dwell time of the instrumental system, the theoretically predicted retention times agree well with those experimentally determined.