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Spectral and Electrochemical Investigation of Intercalations of Adrenaline and CT‐DNA
Author(s) -
Zheng SaiJing,
Lin XiangQin
Publication year - 2003
Publication title -
chinese journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.28
H-Index - 41
eISSN - 1614-7065
pISSN - 1001-604X
DOI - 10.1002/cjoc.20030210712
Subject(s) - chemistry , dna , intercalation (chemistry) , minor groove , molecule , nucleotide , biophysics , biochemistry , inorganic chemistry , organic chemistry , gene , biology
A strong interaction between double stranded calf‐thymus DNA (ds‐DNA) and adrenaline in solution, but no interaction between single stranded calf‐thymus DNA (ss‐DNA) and adrenaline was observed by the use of UV‐visible spectroscopy and voltametric techniques. It is suggested that the interaction leads to an intercalation of adrenaline molecules into the groove of ds‐DNA and the formation of ds‐DNA (adrenaline), complex. The binding site size of the interaction of adrenaline with CT‐DNA in nucleotide phosphate [NP] has been determined as 25. The interaction of different concentration adrenaline with DNA modified GCE shows that the DNA modified GCE can be a good tool to detect lower concentration a‐drenaline.