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Microcalorimetric Studies of the Toxic Action of La 3+ on Halobacterium Halobium R1 Growth
Author(s) -
Peng Liu,
Yi Liu,
YouGui Chen,
SongSheng Qu,
ZhiXiong Xie,
RuMing Zhao,
Ping Shen
Publication year - 2003
Publication title -
chinese journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.28
H-Index - 41
eISSN - 1614-7065
pISSN - 1001-604X
DOI - 10.1002/cjoc.20030210623
Subject(s) - halobacterium , chemistry , isothermal microcalorimetry , halobacteriaceae , transmission electron microscopy , ampoule , growth inhibition , halobacterium salinarum , nuclear chemistry , bacteriorhodopsin , cell growth , biochemistry , nanotechnology , organic chemistry , materials science , enthalpy , physics , membrane , quantum mechanics
A microcalorimetric technique was used to evaluate the influence of La 3+ on Halobacterium halobium Rl growth. By means of LKB‐2277 bioactivity monitor, ampoule method at 37 °C, the thermogenic curves of Halobacterium halobium R1 growth were obtained. In order to analyze the results, the maximum power P m and the growth rate constants k were determined, showing that values of P m and k are linked to the concentration of La 3+ . Addition of low concentration of La 3+ can cause a decrease of the maximum heat production and growth rate constant. However, high concentration of La 3+ may promote growth of Halobacterium halobium R1, but at much higher concentration of La 3+ , the growth of Halobacterium halobium R1 is inhibited again. For comparison, the shapes of Halobacterium halobium R1 cell were observed by means of transmission electron microscope. According to the thermogenic curves and TEM photos of Halobacterium halobium R1 under different conditions, it is clear that metabolic mechanism of Halobacterium halobium R1 growth is changed with the addition of La 3+ .