Premium
A Novel Fluorescent Reagent for Analysis of Hydrogen Peroxide
Author(s) -
SuYing Dong,
MeiHong Su,
LiHua Nie,
HuiMin Ma
Publication year - 2003
Publication title -
chinese journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.28
H-Index - 41
eISSN - 1614-7065
pISSN - 1001-604X
DOI - 10.1002/cjoc.20030210219
Subject(s) - chemistry , hydrogen peroxide , reagent , detection limit , fluorescence , linear range , quinoline , relative standard deviation , peroxide , chromatography , nuclear chemistry , organic chemistry , quantum mechanics , physics
8‐(4,6‐Dichloro‐1,3,5‐triazinoxy)quinoline (DTQ) was evaluated as a new fluorescent reagent for determining hydrogen peroxide. It was found that the fluorescence intensity of DTQ in alkaline medium could be dramatically enhanced upon addition of H 2 Q 2 . Based on this effect, a simple and selective method for the spectrofluorimetric determination of hydrogen peroxide was established. The relative standard deviation of the method was found to be 1.1% for 9 replicate determinations of a 4.6 × 10– 6 mol/L hydrogen peroxide solution. The linear range was 2.3 × 10– 7 —2.3 × 10– 5 mol/L with a detection limit of 2.2 × 10– 8 mol/L ( S/N = 3). The method was attempted to determine hydrogen peroxide in synthetic human serum samples with satisfactory results.