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Flow‐injection Chemiluminescence Determination of Reserpine in Medicine and Biological Fluids with Controlled‐Reagent‐Release Technology
Author(s) -
ZhengHua Song,
Ni Zhang
Publication year - 2003
Publication title -
chinese journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.28
H-Index - 41
eISSN - 1614-7065
pISSN - 1001-604X
DOI - 10.1002/cjoc.20030210217
Subject(s) - chemistry , reserpine , reagent , chemiluminescence , luminol , detection limit , chromatography , elution , flow injection analysis , pharmacology , organic chemistry , medicine
A sensitive and rapid chemiluminescence (CL) flow injection with controlled‐reagent‐release technology for the determination of reserpine was proposed. The CL reagents, luminol and dichromate, used in this sensor, were all immobilized on anion‐exchange resin. Through injection of 100 μL of water, the reagents on the anion‐exchange resin column were eluted and in the presence of reserpine, the CL intensity was decreased, by which reserpine could be sensed. Reserpine was quantified by measuring the decrement of CL intensity, which was observed linear with the logarithm of reserpine concentration in the range of 1.0–500.0 ng/mL, and the limit of detection was 0.4 ng/mL (3σ) with a relative standard deviation of less than 3.0%. The proposed procedure was applied in the assay of reserpine in pharmaceutical preparation and biological fluids without any pre‐treatment process and with sampling frequencies of 72 times per hour.