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Structural Elucidation and Antitumor Activity of Polysaccharide AMP‐1 from Atractylodis macrocephalae K.
Author(s) -
JunJie Shan,
Wd Ke,
JunE Deng,
GengYuan Han
Publication year - 2003
Publication title -
chinese journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.28
H-Index - 41
eISSN - 1614-7065
pISSN - 1001-604X
DOI - 10.1002/cjoc.20030210120
Subject(s) - chemistry , mannose , polysaccharide , galactose , fractionation , residue (chemistry) , size exclusion chromatography , proton nmr , chromatography , molar ratio , capillary electrophoresis , stereochemistry , biochemistry , enzyme , catalysis
A polysaccharide named as AMP‐1 was isolated from the root of Atractylodis macrocephalae Koidz , and purified by ethanol fractionation and gel filtration. The homogeneity of AMP‐1 was determined by HPLC and capillary electrophoresis that gave a single peak. AMP‐1 is composed of galactose and mannose in a molar ratio of 1.0:1.9. Its molecular weight is 3.8 × 10 3 . The structure of ghycan was elucidated by IR, 1 H NMR, 13 C NMR and methylation analysis, and on the basis of the results was suggested that AMP‐1 contain a backbone of β. (1–2)‐D‐galactose residues with branches of single β ‐D ‐mannose residue being substituted at the O ‐6. Bioactivity assay of AMP‐1 showed that it could inhibit growth of Sarcoma 180 and Lewis pulmonary carcinoma implanted in mice.