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Resonance Rayleigh Scattering Spectra of Thorium (IV)‐bisazo Dye of Chromotropic Acid‐protein Systems and Their Analytical Applications
Author(s) -
Fan U,
ShaoPu Liu,
DaCheng Yang,
HongQun Luo
Publication year - 2003
Publication title -
chinese journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.28
H-Index - 41
eISSN - 1614-7065
pISSN - 1001-604X
DOI - 10.1002/cjoc.20030210114
Subject(s) - chromotropic acid , chemistry , thorium , chelation , resonance (particle physics) , detection limit , nuclear chemistry , selectivity , analytical chemistry (journal) , chromatography , inorganic chemistry , reagent , organic chemistry , materials science , physics , uranium , particle physics , metallurgy , catalysis
In acidic medium, thorium (IV) can react with a bisazo dye of chromotropic adds such as arsenazo HI (AA in), arsenazo M (AA M), chlorophosphonazo III (CPA III) and chlorosulphonphenol S (CSP S) to form an anionic chelate which further interacts with some proteins to produce a complex. This results in a significant enhancement of intensity of the resonance Rayleigh scattering (RRS) and the appearance of a new RRS spectrum. There are a few obvious RRS peaks in the range of 400–470 nm and the most intensive peak of them is located at 470 nm. The intensity of RRS is directly proportional to the concentration of protein in the range of 0—l.öμg‐mL −1 for Th(IV)‐CPA III system, 0–2.8 μg‐mL −1 for Th(IV)‐AA M system, 0–2.0 μg·mL −1 for Th(IV)‐AA III system and 0–0.28 μg·mL −1 for Th(IV)‐CSP S system, respectively. The detection limits for BSA (3s̀) are 10.7 ng·mL −1 for Th (IV)‐CPA III, 6.3 ng·mL −1 for Th(IV)‐CSP S, 13.6 ng·mL −1 for Th(IV)‐AA III and 22.1 ng·mL −1 for Th(IV)‐AA M, respectively. This new RRS method has high sensitivity and fairly good selectivity and can be applied to the direct determination of proteins in human serum with satisfactory results.