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Protective Effects of Resveratrol and its Analogues against Free Radical‐Induced Oxidative Hemolysis of Red Blood Cells †
Author(s) -
Fang JianGuo,
Lu Man,
Ma LanPing,
Yang Li,
Wu LongMin,
Liu ZhongLi
Publication year - 2002
Publication title -
chinese journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.28
H-Index - 41
eISSN - 1614-7065
pISSN - 1001-604X
DOI - 10.1002/cjoc.20020201126
Subject(s) - hemolysis , chemistry , resveratrol , hydrochloride , oxidative damage , oxidative phosphorylation , red blood cell , in vitro , stereochemistry , biochemistry , oxidative stress , immunology , biology
The in vitro oxidative hemolysis of human red blood cells (RBCs) was used as a model to study the free radical‐induced damage of biological membranes and the protective effect of resveratrol (3,5,4′‐trihydroxy‐trans‐stilbene, 1) and its analogues, i. e ., 4‐hydroxy‐ trans ‐stilbene (2), 3, 5‐ dihydroxytrans ‐stilbene (3), 3,4‐dihydroxy‐ trans ‐stilbene (4), 4,4′‐dihydroxy‐ trans ‐stilbene (5) and 2, 4, 4′‐trihydroxy‐ trans ‐stilbene (6). The hemolysis of RBCs was induced by a water‐soluble free radical initiator 2, 2′‐azobis (2‐amidinopropane hydrochloride) (AAPH). It was found that addition of AAPH at 37 °C to the suspension of RBCs caused fast hemolysis after a short period of inhibition period, and addition of 1–6 significantly suppressed the hemolysis. Compound 4 which bears an ortho ‐dihydroxyl functionality showed much more effective anti‐hemolysis activity than that of resveratrol and the other analogues.