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Bioelectrochemical Response and Kinetics of Choline Oxidase Entrapped in Polyaniline‐Polyacrylonitrile Composite Film
Author(s) -
Xue HuaiGuo,
Shen ZhiQuan
Publication year - 2002
Publication title -
chinese journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.28
H-Index - 41
eISSN - 1614-7065
pISSN - 1001-604X
DOI - 10.1002/cjoc.20020200814
Subject(s) - polyacrylonitrile , choline oxidase , chemistry , polyaniline , electrode , choline , aniline , electrochemistry , immobilized enzyme , glucose oxidase , kinetics , inorganic chemistry , chemical engineering , polymer chemistry , organic chemistry , enzyme , biochemistry , polymer , polymerization , engineering , acetylcholinesterase , physics , quantum mechanics
A novel choline oxidase electrode was constructed by entrapping choline oxidase into polyaniline‐polyacrylonitrile composite film. The enzyme film was prepared by in situ electropolymerization of aniline into porous polyacrylonitrile‐coated platinum electrode in the presence of choline oxidase. The enzyme electrode exhibited sensitive and stable electrochemical response to choline. The kinetics analysis showed that the mass transport is partially rate‐limiting. The influences of pH, applied potential and temperature on the response of the enzyme electrode were also described.