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Microcalorimetric Study of the Biological Effects of Zn 2+ on Bacillus thuringiensis Growth
Author(s) -
Yao Jun,
Liu Yi,
Gao ZhenTing,
Liu Peng,
Sun Ming,
Zou Xue,
Qu SongSheng,
Yu ZiNiu
Publication year - 2002
Publication title -
chinese journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.28
H-Index - 41
eISSN - 1614-7065
pISSN - 1001-604X
DOI - 10.1002/cjoc.20020200808
Subject(s) - bacillus thuringiensis , chemistry , isothermal microcalorimetry , growth rate , bacterial growth , bacillaceae , metabolism , biochemistry , bacteria , enthalpy , thermodynamics , bacillus subtilis , genetics , physics , geometry , mathematics , biology
A microcalorimetric technique was used to investigate the influence of Zn 2+ on the growth metabolism of Bacillus thuringiensis . LKB‐2277 Bioactivity Monitor was employed to obtain the power‐time curves, from which the maximum peak‐heat output power ( P max ) in the log phase, the growth rate constants ( k ), the inhibitory ratios ( I ), the generational time ( t G ) and the total heat effect ( Q total ) in 23 h for the growth metabolism of Bacillus thuringiensis at 28 °C can be evaluated. The results indicate that the concentration of Zn 2+ affects its growth obviously. Low concentration (0–50 μg/mL) of Zn 2+ promotes the growth of Bacillus thuringiensis while high concentration (50–500 μg/mL) of Zn 2+ inhibits its growth. When the concentration reached up to 600 μg/mL, it can not grow at all.