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Enantiomer Separation of α‐Dimethyl Dicarboxylate Biphenyl and Related Biphenyl Compounds by Normal Phase HPLC on Polysaccharide Based Chiral Stationary Phases
Author(s) -
Liu YueQi,
Han XiaoQian,
Qi BangFeng,
Liu ChunHui,
Li YongMin,
Chen LiRen
Publication year - 2002
Publication title -
chinese journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.28
H-Index - 41
eISSN - 1614-7065
pISSN - 1001-604X
DOI - 10.1002/cjoc.20020200708
Subject(s) - chemistry , trifluoroacetic acid , biphenyl , enantiomer , high performance liquid chromatography , chromatography , chiral column chromatography , tris , amylose , cellulose , silica gel , hexane , phase (matter) , organic chemistry , starch , biochemistry
Cellulose tris(4‐methylphenylcarbamate), amylose tris(3,5‐dimethylphenylcarbamate) and amylose tris (phenylcarbamate) were prepared by the method reported by Okamoto and were coated onto an aminopropylated mesoporous spherical silica gel. These final products were used as chiral stationary phases of high performance liquid chromatography for the eighteen structurally related biphenyl compounds. The resolution was made using normal‐phase methodology with a mobile phase consisting of n ‐hexane‐alcohol (ethanol, 1‐propanol, 2‐propanol or 1‐butanol). The effects of various aliphatic alcohols in the mobile phase were studied. The structural features of the solutes that influence their k ′were discussed. A dominant effect of trifluoroacetic acid on chiral separation of acidic solutes was noted.