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Spectroscopic and Spectroelectrochemical Studies of Interaction of Nile Blue with DNA
Author(s) -
Zhao GuangChao,
Zhu JunJie,
Chen HongYuan,
Wang XueMei,
Lu ZuHong
Publication year - 2002
Publication title -
chinese journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.28
H-Index - 41
eISSN - 1614-7065
pISSN - 1001-604X
DOI - 10.1002/cjoc.20020200112
Subject(s) - chemistry , intercalation (chemistry) , dna , nile blue , binding constant , nile red , binding site , stereochemistry , crystallography , biochemistry , inorganic chemistry , fluorescence , physics , quantum mechanics
Nile Blue can strongly bind to DNA and its affinity for DNA has been investigated by spectroscopy and spectroelectrochemistry. At low DNA concentrations, Nile Blue can bind to DNA(per nucleotide phosphate) to form a 1:1 association complex with the binding constant of 4.7 × 10 4 L/mol and the major binding model of Nile Blue to DNA is “electrostatic binding”. However, the major binding model changes into “intercalative binding” at high DNA concentrations. In order to confirm which part of Nile Blue intercalating into DNA, the inclusion action of β‐cydodextrin (β‐CD) has been used to study the interaction. Nile Blue can be included into the hydrophobic cavity of β‐CD to form an inclusion complex with a stability constant of 1.9 × 10 3 L/mol. The experimental results indicate that the naphthalene ring part of Nile Blue is included into β‐CD's hydrophobic cavity and the inclusion complex decomposes at high DNA concentrations. It may be deduced reasonably that the naphthalene ring part of Nile Blue molecule intercalates into DNA helix Strand.