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Electrochemical study on interaction of vincristine with tubulin
Author(s) -
Yu Yong,
Li QiLong
Publication year - 2001
Publication title -
chinese journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.28
H-Index - 41
eISSN - 1614-7065
pISSN - 1001-604X
DOI - 10.1002/cjoc.20010191114
Subject(s) - chemistry , electrochemistry , dimer , tubulin , tris , linear sweep voltammetry , vincristine , buffer solution , cyclic voltammetry , voltammetry , binding constant , peak current , nuclear chemistry , analytical chemistry (journal) , chromatography , electrode , organic chemistry , binding site , biochemistry , microtubule , medicine , surgery , chemotherapy , biology , microbiology and biotechnology , cyclophosphamide
In Tris (0.005 mol/L)‐NaCl (0.05 mol/L) buffer solution (pH = 7.10), keeping temperature at 37°C, a highly sensitive reduction peak of the antitumor agent was obtained by linear sweep voltammetry. The peak potential is‐1.56 V ( vs . SCE). The peak current is proportional to the concentration of vincristine over the range of 2.1 ± 10 −7 ‐4.2 ± 10 −6 mol/L with the detection limit of 1.0 ± 10 −7 mol/L. The behavior of the binding of vincristine to tubulin was studied. The results showed that the reaction of tubulin dimer with vincristine formed an electrochemically active complex to be 1:2. Its stability constant is 2.5 ± 10 14 . The reduction process of the complex is irreversible with adsorptive characteristics.