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Electrostatic Layer‐by‐Layer Assembly of Polycation and DNA Multilayer Films by Real‐time Surface Plasmon Resonance Technique
Author(s) -
Pei RenJun,
Cui XiaoQiang,
Yang XiuRong,
Wang ErKang
Publication year - 2001
Publication title -
chinese journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.28
H-Index - 41
eISSN - 1614-7065
pISSN - 1001-604X
DOI - 10.1002/cjoc.20010190421
Subject(s) - surface plasmon resonance , chemistry , adsorption , layer (electronics) , substrate (aquarium) , kinetics , layer by layer , surface plasmon , resonance (particle physics) , langmuir , chemical engineering , analytical chemistry (journal) , electrostatics , polyelectrolyte , chloride , plasmon , chromatography , nanoparticle , optoelectronics , organic chemistry , materials science , atomic physics , oceanography , physics , quantum mechanics , engineering , geology , polymer
Abstract The assembly of alternating DNA and positively charged poly‐(dimethyldiallylammonium chloride) (PDDA) multilayer films by electrostatic layer‐by‐layer adsorption has been studied. Real time surface plasmon resonance (BIAcore) technique was used to characterize and monitor the formation of multilayer films in solution in real time continuously. The results indicate that the uniform multilayer can be obtained on the poly‐(ethylenimine) (PEI) coated substrate surface. The kinetics of the adsorption of DNA on PDDA surface was also studied by real‐time BIAcore technique, and the observed rate constant was calculated using a Langmuir model ( k obs = (1.28 ± 0.08) × 10 −2 s −1 ).