Premium
Microcalorimetric Studies on Thermochemical Characteristics of Escherichia coli NCTC 10418 Aerobic Growth Metabolism in Basic Media
Author(s) -
Liu Yi,
Gao ZhenTing,
Wang Hong,
Qu SongSheng
Publication year - 2001
Publication title -
chinese journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.28
H-Index - 41
eISSN - 1614-7065
pISSN - 1001-604X
DOI - 10.1002/cjoc.20010190307
Subject(s) - metabolism , chemistry , escherichia coli , cell metabolism , endogeny , biochemistry , steady state (chemistry) , biophysics , biology , gene
By using an LKB‐2277 bioactivity monitor, cycle‐flow mode, the thermogenic curve of metabolism of Escherichia coü NCTC 10418 growth at 37°C in basic media was determined. The experimental results indicate that the relationship between cell concentration and power output can be characterized by the equations C = k'P + a dC/dP 0 = KC, n = 1 where P is the power output (μW), C is cell concentration (mg/mL), P 0 is the power output produced by the metabolism of one unit of cell ( P 0 = P/0.6C ), the order of metabolism n is 1, and k', a and K are constants which depend on the culture condition and phylogenic state of the cells. These equations are different from those of the non‐growth metabolism of resting cells and endogenous metabolism of cells. For different kinds of metabolism of cells, the order of metabolism, n , is different. For endogenous metabolism, n = 0, for growth metabolism, n = 1, and for non‐growth metabolism of resting cells, n = 2. The equations are general thermochemical equations for the various different kinds of metabolism of cells.