Premium
LC‐MS analysis of the formed peptides from N‐(O, O‐ diiso‐propyl) phosphoryl aspartic acid
Author(s) -
Hu JianJun,
Ju Yong,
Zhao YuFen
Publication year - 2000
Publication title -
chinese journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.28
H-Index - 41
eISSN - 1614-7065
pISSN - 1001-604X
DOI - 10.1002/cjoc.20000180624
Subject(s) - chemistry , aspartic acid , peptide , nucleoside , selectivity , nucleotide , high performance liquid chromatography , chromatography , adenosine , mass spectrometry , stereochemistry , amino acid , organic chemistry , biochemistry , catalysis , gene
LC‐ESI‐MS method was used to analyze the formed di and tri‐peptide in the reaction system of N‐ ( O, O ‐diisopropyl) phosphoryl aspartic acid and adenosine. Cluster ions of the peptides were given in the ESI‐MS. The structures of these small peptides were confirmed by LC‐MS‐MS analysis. Compared with the traditional HPLC‐UV detection, this method showed good sensitivity and selectivity for peptide in the presence of compounds with strong UV absorption, such as nucleoside and nucleotide.