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Influence of preparation conditions on properties of chemical modified nylon affinity membrane (used for γ‐globulin adsorption)
Author(s) -
HongYu Gan,
ZhenHua Shang,
JunDe Wang,
XueLiang Liu
Publication year - 2000
Publication title -
chinese journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.28
H-Index - 41
eISSN - 1614-7065
pISSN - 1001-604X
DOI - 10.1002/cjoc.20000180411
Subject(s) - chemistry , membrane , microporous material , adsorption , polyamide , polymer chemistry , hydrolysis , nylon 6 , chromatography , ligand (biochemistry) , organic chemistry , polymer , biochemistry , receptor
Microporous polyamide membrane (Nylon 6, PA) was modified by add hydrolysis and then coupled with hydroxyethyl cellulose (HEC) to amplify reactive groups and reduce nonspecific interaction with proteins. 1,6‐Diaminohexane (DAH) as space arm and phenylalanine (Phe) as ligand were immobilized onto nylon membrane through the activation of s‐triazine trichloride (CyCl 3 ). The affinity membrane obtained can be used to adsorb γ‐globulin specifically. The optimum conditions of preparing affinity membrane and adsorbing bovine γ‐globulin were studied.