An improved ELISA for the determination of southern bean mosaic virus with linear sweep voltammetry detection based on new system of PAP‐H 2 O 2 ‐HRP | Zendy

ShuSheng Zhang | Zendy; Kui Jiao | Zendy; HongYuan Chen | Zendy; ChengLiang Zhang | Zendy; ZuoFang Zhang | Zendy

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An improved ELISA for the determination of southern bean mosaic virus with linear sweep voltammetry detection based on new system of PAP‐H 2 O 2 ‐HRP

Author(s) -

ShuSheng Zhang,

Kui Jiao,

HongYuan Chen,

ChengLiang Zhang,

ZuoFang Zhang

Publication year - 2000

Publication title -

chinese journal of chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.28

H-Index - 41

eISSN - 1614-7065

pISSN - 1001-604X

DOI - 10.1002/cjoc.20000180110

Subject(s) - chemistry , detection limit , linear sweep voltammetry , horseradish peroxidase , linear range , voltammetry , cyclic voltammetry , buffer solution , analytical chemistry (journal) , peroxidase , chromatography , nuclear chemistry , enzyme , electrochemistry , electrode , biochemistry

An improved enzyme‐linked immunosorbent assay (ELISA) for the determination of southern bean mosaic virus (SBMV) with linear sweep voltammetry based on a new system of p‐ aminophenol (PAP)‐H 2 Q 2 ‐horseradish peroxidase (HRP) has firstly been developed. The enzymatic product 3‐[(4‐hydroxyphenyl)amino]‐4‐(2‐amino‐5‐hydroxvphenyl)‐6‐[(4‐hydroxyphenyl)imino]‐2,4‐cyclohexadiene‐1‐one, produced from the oxidation of PAP with H 2 O 2 catalyzed by HRP, yielded a sensitive linear sweep voltammetric response at −0.45 V ( vs . SCE) in Britton‐Robinson (BR) buffer solution. Based on the voltammetric peak, HRP can be measured with a detection limit of 0.4 mU/L and a linear range of 1.0–1.0×10 2 mU/ L. The detection limit for the SBMV is 8.0 ng/mL and the highest dilution ratio for the detection of infected leaf sap is 1: 1.5×10 5 .

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