Premium
Structure determination of glycopeptide of p‐momorcharin
Author(s) -
Li YiLi,
Ma Kan,
Wu HouMing,
Ye GuoJie,
Jin ShanWei,
Wang Yu
Publication year - 1999
Publication title -
chinese journal of chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.28
H-Index - 41
eISSN - 1614-7065
pISSN - 1001-604X
DOI - 10.1002/cjoc.19990170513
Subject(s) - glycopeptide , chemistry , pronase , momordica , oligosaccharide , biochemistry , glycoprotein , amino acid , sugar , glycosylation , carbohydrate , amino sugar , stereochemistry , trypsin , enzyme , medicine , traditional medicine , antibiotics
The structure of the N‐linked oligosaccharide chain of β‐momorcharin, a ribosome‐inactivating protein from the seeds of Momordica charantia Linn (Cucurbitaceae) was determined. A glycopeptide liberated by pronase digestion of the glycoprotein was subjected to amino acid and neutral carbohydrate analysis to establish the composition of amino acid and sugar residues. The sequences and glycosylation linkages of the sugar and amino acid residues in the glycopeptide were determined as Manal‐6(Xylβ1‐2)‐Manβ1‐4GlcNAcβ1‐4(Fucal‐3)‐GlcNAc‐Asn‐Leu by 2D‐NMR spectroscopy and FAB‐MS data.